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antibodies against dnmt3a  (Novus Biologicals)


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    Structured Review

    Novus Biologicals antibodies against dnmt3a
    Antibodies Against Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dnmt3a/HES-1+Antibody+(OTI4H1)/pm42014700-311-9-13
    Average 90 stars, based on 12 article reviews
    antibodies against dnmt3a - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Saline:

    Article Title: Mitochondrial genome undergoes de novo DNA methylation that protects mtDNA against oxidative damage during the peri-implantation window
    Article Snippet: The proteins were separated on 10% acrylamide gels containing 0.1% SDS and then transferred onto hydrophobic polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% non-fat dried milk in Tris-buffered saline (TBS) containing 0.1% Tween-20 for 2 h at RT and then incubated overnight at 4 °C with diluted antibodies: anti-DNMT3A and anti-DNMT3B (Imgenex), anti–VDAC, anti-β-Tubulin, and antiH3K27me3, followed by three washes with TBS containing 0.1% Tween-20 for 20 min. .. Secondary antibodies were incubated for 1.5 h, and protein bands were detected using an ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA).

    Article Title: Prenatal lipopolysaccharide exposure programs cardiac fibrosis via dysregulating of connexin 43 in offspring rats
    Article Snippet: .. The membranes were blocked with 5% non-fat dry milk (Santa Cruz Biotechnology. sc-2325) diluted in Tris-buffered saline with Tween-20 (TBST) at room temperature for 1 h, then incubated overnight at 4°C with the following primary antibodies: anti-Cx43 (1:1,000; Abcam. ab11370), anti-microtubule-associated protein-1 light-chain (LC3; 1:1,000; Santa Cruz Biotechnology, Inc. sc-271625), anti-DNMT1 (1:500; Novus Biologicals, LLC; cat. no. NB100-56519), anti-DNMT3A (1:1,000; Novus Biologicals, LLC. .. NB120-13888), anti-DNMT3B (1:1,000; Novus Biologicals, LLC.

    Incubation:

    Article Title: Mitochondrial genome undergoes de novo DNA methylation that protects mtDNA against oxidative damage during the peri-implantation window
    Article Snippet: The proteins were separated on 10% acrylamide gels containing 0.1% SDS and then transferred onto hydrophobic polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% non-fat dried milk in Tris-buffered saline (TBS) containing 0.1% Tween-20 for 2 h at RT and then incubated overnight at 4 °C with diluted antibodies: anti-DNMT3A and anti-DNMT3B (Imgenex), anti–VDAC, anti-β-Tubulin, and antiH3K27me3, followed by three washes with TBS containing 0.1% Tween-20 for 20 min. .. Secondary antibodies were incubated for 1.5 h, and protein bands were detected using an ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA).

    Article Title: Prenatal lipopolysaccharide exposure programs cardiac fibrosis via dysregulating of connexin 43 in offspring rats
    Article Snippet: .. The membranes were blocked with 5% non-fat dry milk (Santa Cruz Biotechnology. sc-2325) diluted in Tris-buffered saline with Tween-20 (TBST) at room temperature for 1 h, then incubated overnight at 4°C with the following primary antibodies: anti-Cx43 (1:1,000; Abcam. ab11370), anti-microtubule-associated protein-1 light-chain (LC3; 1:1,000; Santa Cruz Biotechnology, Inc. sc-271625), anti-DNMT1 (1:500; Novus Biologicals, LLC; cat. no. NB100-56519), anti-DNMT3A (1:1,000; Novus Biologicals, LLC. .. NB120-13888), anti-DNMT3B (1:1,000; Novus Biologicals, LLC.

    Immunoprecipitation:

    Article Title: Induction of FAM46C expression mediated by DNMT3A downregulation is involved in early-onset preeclampsia through gene body methylation.
    Article Snippet: Background: Aberrant methylation of genomic DNA has been found in preeclamptic placentas, which is characterized by elevated DNA methylation and hypermethylation of gene body regions, but the underlying mechanism is not yet fully understood.. Methods: Global DNA methylation was assessed through ELISA and HPLC.. The methylation sites were detected using the Illumina Human Methylation 450 K Microarray.

    Western Blot:

    Article Title: Ginseng mediates its anticancer activity by inhibiting the expression of DNMTs and reactivating methylation-silenced genes in colorectal cancer.
    Article Snippet: Developing safe and effective therapeutic modalities remains a critical challenge for improving the prognosis of patients with colorectal cancer (CRC).. In this regard, targeting epigenetic regulation in cancers has recently emerged as a promising therapeutic approach.. Since several natural compounds have recently been shown to be important epigenetic modulators, we hypothesized that Ginseng might exert its anticancer activity by regulating DNA methylation alterations in CRC.

    Protease Inhibitor:

    Article Title: Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions
    Article Snippet: Protein concentration of the nuclear extract was determined using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). .. Nuclear extracts containing 200 μg of protein were reacted with anti-DNMT3a (64B1446, Novus Biologicals, Centennial, CO, United States) or anti-DNMT3b (52A1018, Novus Biologicals) antibody in the presence of 0.1% NP-40 and protease inhibitor cocktail (Nacalai Tesque) at 4°C overnight with rotation. .. Dynabeads Protein G (Veritas, Thermo Fisher Scientific) were suspended in TNEN buffer (20 mM Tris-HCl, pH7.5 containing 150 mM NaCl, 1 mM EDTA, and 0.1% (v/v) NP-40), added to above mixture, and incubated at 4°C for 1 h with rotation.

    Article Title: Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions.
    Article Snippet: Frontiers in Molecular Biosciences frontiersin.org03 .. Nuclear extracts containing 200 μg of protein were reacted with anti-DNMT3a (64B1446, Novus Biologicals, Centennial, CO, United States) or anti-DNMT3b (52A1018, Novus Biologicals) antibody in the presence of 0.1% NP-40 and protease inhibitor cocktail (Nacalai Tesque) at 4°C overnight with rotation. .. Dynabeads Protein G (Veritas, Thermo Fisher Scientific) were suspended in TNEN buffer (20 mM Tris-HCl, pH7.5 containing 150 mM NaCl, 1 mM EDTA, and 0.1% (v/v) NP-40), added to above mixture, and incubated at 4°C for 1 h with rotation.



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    Image Search Results


    GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

    Journal: 3 Biotech

    Article Title: Gandouling protects against hepatic fibrosis in Wilson disease through the lncRNA-SNHG7/miR-29b/DNMT3A pathway

    doi: 10.1007/s13205-026-04769-0

    Figure Lengend Snippet: GDL treatment improves liver function and fibrosis-related serological indices in WD model rats. A Serum levels of hyaluronic acid (HA), laminin (LN), type III pro-collagen (PC-III), collagen IV (C-IV), aspartate aminotransferase (ALT), and alanine aminotransferase (AST) in each group ( n = 6). B Hepatic copper content across different groups ( n = 6). C Protein expression of α-SMA and collagen I in rat liver tissues, as determined by Western blotting ( n = 3). D Protein expression of α-SMA and collagen I in LX-2 cells treated with CuSO₄ and GDL-containing serum ( n = 3). GAPDH was used as a loading control. Data are presented as means ± SD. Statistical significance was determined by one-way ANOVA with Bonferroni’s post hoc test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. model group; * p < 0.05, ** p < 0.01 vs. CuSO 4 group

    Article Snippet: The membranes were then incubated overnight with antibodies against DNMT3A (Bioss, Item No. bs-23029R, 1:2000, Rabbit, 1:1000, Rabbit), p-DNMT3A (Bioss, Item No. bs-14399R, 1:2000, Rabbit), α-SMA (Bioss, Item No. Bsm-33187 M, 1:2000, Mouse; No. bs-0189R, 1:1000, Rabbit), Collagen I (Bioss, Item No. bs-7158R, 1:1000, Rabbit; No. AB260043 , 1:1000, Rabbit), Beclin-1 (Abcam, Item No. ab210498, 1:2000, Rabbit; No. ab62472, 1:1000, Rabbit), LC3B (CST, Item No. 3868s, 1:1000, Rabbit; No. 43566 S, 1:1000, Rabbit), and p62 (Bioss, Item No. bs-2951R, 1:1000, Rabbit) at 4°C with gentle shaking.

    Techniques: Expressing, Western Blot, Control