antibodies against dnmt3a (Novus Biologicals)
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Antibodies Against Dnmt3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+dnmt3a/HES-1+Antibody+(OTI4H1)/pm42014700-311-9-13
Average 90 stars, based on 12 article reviews
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Saline:Article Title: Mitochondrial genome undergoes de novo DNA methylation that protects mtDNA against oxidative damage during the peri-implantation window Article Snippet: The proteins were separated on 10% acrylamide gels containing 0.1% SDS and then transferred onto hydrophobic polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% non-fat dried milk in Tris-buffered saline (TBS) containing 0.1% Tween-20 for 2 h at RT and then incubated overnight at 4 °C with diluted antibodies: Article Title: Prenatal lipopolysaccharide exposure programs cardiac fibrosis via dysregulating of connexin 43 in offspring rats Article Snippet: .. The membranes were blocked with 5% non-fat dry milk (Santa Cruz Biotechnology. sc-2325) diluted in Tris-buffered saline with Tween-20 (TBST) at room temperature for 1 h, then incubated overnight at 4°C with the following primary antibodies: anti-Cx43 (1:1,000; Abcam. ab11370), anti-microtubule-associated protein-1 light-chain (LC3; 1:1,000; Santa Cruz Biotechnology, Inc. sc-271625), anti-DNMT1 (1:500; Novus Biologicals, LLC; cat. no. NB100-56519), Incubation:Article Title: Mitochondrial genome undergoes de novo DNA methylation that protects mtDNA against oxidative damage during the peri-implantation window Article Snippet: The proteins were separated on 10% acrylamide gels containing 0.1% SDS and then transferred onto hydrophobic polyvinylidene fluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% non-fat dried milk in Tris-buffered saline (TBS) containing 0.1% Tween-20 for 2 h at RT and then incubated overnight at 4 °C with diluted antibodies: Article Title: Prenatal lipopolysaccharide exposure programs cardiac fibrosis via dysregulating of connexin 43 in offspring rats Article Snippet: .. The membranes were blocked with 5% non-fat dry milk (Santa Cruz Biotechnology. sc-2325) diluted in Tris-buffered saline with Tween-20 (TBST) at room temperature for 1 h, then incubated overnight at 4°C with the following primary antibodies: anti-Cx43 (1:1,000; Abcam. ab11370), anti-microtubule-associated protein-1 light-chain (LC3; 1:1,000; Santa Cruz Biotechnology, Inc. sc-271625), anti-DNMT1 (1:500; Novus Biologicals, LLC; cat. no. NB100-56519), Immunoprecipitation:Article Title: Induction of FAM46C expression mediated by DNMT3A downregulation is involved in early-onset preeclampsia through gene body methylation. Article Snippet: Background: Aberrant methylation of genomic DNA has been found in preeclamptic placentas, which is characterized by elevated DNA methylation and hypermethylation of gene body regions, but the underlying mechanism is not yet fully understood.. Methods: Global DNA methylation was assessed through ELISA and HPLC.. The methylation sites were detected using the Illumina Human Methylation 450 K Microarray. Western Blot:Article Title: Ginseng mediates its anticancer activity by inhibiting the expression of DNMTs and reactivating methylation-silenced genes in colorectal cancer. Article Snippet: Developing safe and effective therapeutic modalities remains a critical challenge for improving the prognosis of patients with colorectal cancer (CRC).. In this regard, targeting epigenetic regulation in cancers has recently emerged as a promising therapeutic approach.. Since several natural compounds have recently been shown to be important epigenetic modulators, we hypothesized that Ginseng might exert its anticancer activity by regulating DNA methylation alterations in CRC. Protease Inhibitor:Article Title: Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions Article Snippet: Protein concentration of the nuclear extract was determined using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). .. Nuclear extracts containing 200 μg of protein were reacted with Article Title: Gut microbiota-dependent adaptor molecule recruits DNA methyltransferase to the TLR4 gene in colonic epithelial cells to suppress inflammatory reactions. Article Snippet: Frontiers in Molecular Biosciences frontiersin.org03 .. Nuclear extracts containing 200 μg of protein were reacted with |
